An Experimental HepG2 Cell Model of Hydrogen Peroxide Induced DNA Oxidative Injury

doi:10.7506/spkx1002-6630-201105012

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (5 ): 55-57.doi: 10.7506/spkx1002-6630-201105012

• Basic Research • Previous Articles Next Articles

An Experimental HepG2 Cell Model of Hydrogen Peroxide Induced DNA Oxidative Injury

HAN Fei，ZHOU Meng-liang

Academy of State Administration of Grain, Beijing 100037, China

Received:2010-07-12 Revised:2011-01-19 Online:2011-03-15 Published:2011-03-03
Contact: HAN Fei E-mail:hf@chinagrain.org

Abstract

Abstract: The oxidative effect of 4 h exposure to H2O2 at different concentrations on HepG2 cells was measured by determining DNA damage by single cell gel electrophoresis technique and assaying MDA content and the activities of SOD and GSH-Px in HepG2 cells. An experimental model of oxidative stress was established based on the optimal concentration of H2O2. The results showed that the optimal H2O2 concentration determined was 100μmol/L and construction of the HepG2 cell model of oxidative injury came to success after 4 h treatment with H2O2 at this concentration.

Key words: H2O2, HepG2 cell, oxidative stress model

CLC Number:

Q505

HAN Fei，ZHOU Meng-liang. An Experimental HepG2 Cell Model of Hydrogen Peroxide Induced DNA Oxidative Injury[J]. FOOD SCIENCE, 2011, 32(5 ): 55-57.

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An Experimental HepG2 Cell Model of Hydrogen Peroxide Induced DNA Oxidative Injury

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